Publications

21. Li M#, Wei Y#, Yin J, Lin L, Zhou Y, Hua G, Cao P, Ang EL, Zhao H*, Yuchi Z*, Zhang Y*. Biochemical and structural investigation of taurine:2-oxoglutarate aminotransferase from Bifidobacterium kashiwanohense. Biochem J. 2019 May 14. doi: 10.1042/BCJ20190206.

20. Xing M#, Wei Y#, Zhou Y, Zhang J, Lin L, Hu Y, Hua G, Nanjaraj A.N., Liu D, Wang F, Guo C, Tong Y, Li M, Liu Y, Ang E, Zhao H*, Yuchi Z*, Zhang Y*. Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria. Nat. Commun. 2019, April 8; DOI: 10.1038/s41467-019-09618-8.

19. Zhang J#, Nanjaraj A.N.#, Lin L, Zhou Y, Hu Y, Hua G, Gao Q, Yuchi Z*, Zhang Y*. Structure of glycerol dehydrogenase (GldA) from Escherichia coli. ACTA CRYSTALLOGR F. 2019, Mar; DOI: 10.1107/S2053230X19000037.

18. Zhou Y#,  Wei Y#, Lin L, Xu T, Ang E, Zhao H*, Yuchi Z*, Zhang Y*. Biochemical and structural investigation of sulfoacetaldehyde reductase from Klebsiella oxytocaJ. Biochem. 2019, Feb; DOI: 10.1042/BCJ20190005.

17. Nanjaraj A.N.#, Hu Y, Li P, Yuchi Z, Chen Y*, Zhang Y*. Cloning and expression of a non-ribosomal peptide synthetase to generate blue rose. ACS Synth. Biol. 2018, Sept; DOI: 10.1021/acssynbio.8b00187.

16. Liu D#, Wei Y#, Liu X, Zhou Y, Jiang L, Yin J, Wang F, Hu Y, Nanjaraj A.N., Liu Y, Ang E, Zhao S*, Zhao H*, Zhang Y*. Indoleacetate decarboxylase is a glycyl radical enzyme catalysing the formation of malodorant skatole. Nature Commun. DOI: 10.1038/s41467-018-06627-x.

15. Guo C#, Hu Y#, Yang C, Nanjaraj Urs AN, Zhang Y*Developing a colorimetric assay for Fe(II)/2-oxoglutarate-dependent dioxygenase. Anal Biochem. 2018, May 1; 548: 109-114.

14. Hua G#, Hu Y#, Yang C, Liu D, Mao Z, Zhang L, Zhang Y*. Characterization of santalene synthases using an inorganic pyrophosphatase coupled colorimetric assay. Anal Biochem. 2018, Apr 15; 547: 26-36.

13. Zhang Y#, Yang C, Dancis A, Nakamaru-Ogiso E. EPR studies of wild type and mutant Dre2 identify essential [2Fe–2S] and [4Fe–4S] clusters and their cysteine ligands. J. Biochem. 2017,  Jan; 161(1): 67-78.

12. Zhang Y#, Li H#, Zhang C, An X, Liu L, Stubbe J, Huang M. Conserved Electron Donor Complex Dre2-Tah18 Is Required for Ribonucleotide Reductase Metallocofactor Assembly and DNA Synthesis. Proc Natl Acad Sci U S A. 2014 Apr 29; 111(17): E1695-704.

11. Yoon H, Knight SA, Pandey A, Pain J, Zhang Y, Pain D, Dancis A. Frataxin-bypassing Isu1: characterization of the bypass activity in cells and mitochondria. J. Biochem. 2014, Apr 1; 459(1): 71-81.

10. Zhang Y, Liu L, Stubbe J. Huang M. Investigation of in vivo roles of the carboxyl terminal tails of the small subunit (ββ’) of S. cerevisiae ribonucleotide reductase: contribution to cofactor formation and inter-subunit association within the active holoenzyme. J Biol Chem. 2013 May 17; 288(20): 13951-9.
Selected as the “Papers of The Week”, highlighted at http://www.jbc.org/content/288/20/13960/suppl/DCAuthor_profile

9. Zhang Y, Liu L, Wu X, An X, Stubbe J, Huang M. Investigation of in vivo diferric tyrosyl radical formation in Saccharomyces cerevisiae Rnr2 protein requirement of Rnr4 and contribution of Grx3/4 and Dre2. J Biol Chem. 2011. Dec 2; 286(48): 41499-509.
Selected as the “Papers of The Week”,  highlighted at http://www.jbc.org/content/286/48/41499/suppl/DCAuthor_profile
http://www.asbmb.org/asbmbtoday/asbmbtoday_article.aspx?id=14939

8. Zhang Y, Stubbe J, Bacillus subtilis Class Ib Ribonucleotide Reductase Is a Dimanganese(III)-Tyrosyl Radical Enzyme. Biochemistry. 2011 Jun 28; 50(25): 5615-23.

7. Mühlenhoff U, Molik S, Godoy JR, Uzarska MA, Richter N, Seubert A, Zhang Y, Stubbe J, Pierrel F, Herrero E, Lillig CH, Lill R. Cytosolic monothiol glutaredoxins function in intracellular iron sensing and trafficking via their bound iron-sulfur cluster. Cell Metab. 2010 Oct 6; 12(4): 373-85.

6. Yoon H, Zhang Y, Pain J, Lyver ER, Lesuisse E, Pain D, Dancis A. Rim2, pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria.  J. Biochem. 2011 Nov 15; 440(1): 137-46.

5. Zhang Y, Lyver, ER, Amutha B, Ohnishi, T, Pain, D, and Dancis, A. Dre2, a conserved eukaryotic protein with both [2Fe-2S] and [4Fe-4S] clusters, functions in cytosolic iron-sulfur protein biogenesis. Mol Cell Biol. 2008 Sept; 28(18): 5569-82.

4. Zhang Y, Lyver, ER, Knight, SAB, Pain, D, Lesuisse, E, and Dancis, A. Mrs3p, Mrs4p, and frataxin provide iron for Fe-S cluster synthesis in mitochondria. J Biol Chem. 2006 Aug 11; 281(32): 22493-502.

3. Zhang Y, Lyver, ER, Knight, SAB, Lesuisse, E, and Dancis, A. Frataxin and mitochondrial carrier proteins, Mrs3p and Mrs4p, cooperate in providing iron for heme synthesis. J Biol Chem. 2005 May 20; 280(20): 19794-807.

2. He Y, Alam SL, Proteasa SV, Zhang Y, Lesuisse E, Dancis A, and Stemmler T.L. Yeast frataxin solution structure, iron binding, and ferrochelatase interaction. Biochemistry. 2004 Dec 28; 43(51): 16254-62.

1. Deshmukh M, May M, Zhang Y, Gabbert KK, Karberg KA, Kranz RG, and Daldal F. Overexpression of ccl1-2 can bypass the need for the putative apocytochrome chaperone CycH during the biogenesis of c-type cytochromes. Mol. Microbiology. 2002 Nov; 46(4)1069-1080.

Patents

  1. 铁和α酮戊二酸依赖性双加氧酶的酶活检测体系和方法(A generally applicable iron and α-oxoglutarate dependent dioxygenase enzyme assay system and assay kit). ZL 2015 1 0897467.5
  2. 催化谷氨酰胺合成靛蓝获得蓝色花卉的转基因方法 (A method to obtain transgenic blue rose by introducing non-ribosomal peptide synthetase catalyzing the formation of indigoidine from glutamine). pending
  3. 一种高产檀香油的重组酵母菌及其构建方法与应用( A genetically engineered baker yeast strain and its construction for high yield biosynthetic santalol oil production). Pending
  4. 半乳糖凝集素-3检测试剂盒(Galectin-3 quantitation kit)ZL 2017 10589093.x
  5. 波形蛋白的体外重折叠方法(A new methodology for Vimentin recombinant protein refolding)ZL 2017 10565618.6